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The macrocycle was purified by reversed-phase HPLC (RP-HPLC), using a Prominence HPLC system (Shimadzu) under linear gradient conditions

Ectopic tumour transplant An experimental tumour that results from the injection of cancer cells into an anatomical site that is different from the one from which the cancer cells were originally derived

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However, this antioxidant effect was significantly reversed by MK2 inhibitor treatment, which increased ROS levels by approximately 40%50% relative to the H 2 O 2 + MAPKAPK2 group (Figure 4C)